Abstract
The application of nanotechnology in regenerative medicine gives opportunity to create 3D scaffolds based on nanofibres with the aim to mimic the structure of native ECM and to serve as environment for cell attachment, proliferation and pertaining to their function. Combining electrospun nanofibres
with adhesive proteins could further improve the biocompatibility of the scaffolds. In this work, we study the influence of alternating current electric fields on the adhesion of albumin (Alb), fibrinogen (FNG) and fibronectin (FN) onto two different poly(ether imide) (PEI-1 and PEI-2) nanofibres. The results are compared with two 2D PEI materials and glass. The data obtained reveal that nanofibrous materials (PEI-1 and PEI-2) adsorb higher amounts of plasma proteins Alb, FNG and FN than conventional 2D polymer materials. This is related to higher surface : volume ratio. Application of low intensity alternating electric fields with frequencies between 1 and 10 Hz results in increased adsorption
of plasma proteins. Frequencies above 10 Hz lead to diminished protein adsorption.
