Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococus fascians

Butschak, G.; Karsten, U.; Schelhaas, U.; Ott, H.; Emmendoerffer, A.; Niemeyer, B.; Helmholz, H.; Goletz, S.

Journalpaper

Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococus fascians

Abstract

In a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, Rhodococcus fascians, were able to induce cytokine release (TNFα) from mouse peritoneal macrophages. Monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (MWN100 kDa) glycoprotein. In addition, methods practicable for its biotechnological preparation and several ELISA variants for its determination were developed.

DOI Link to Publication

URL: https://publications.hereon.de/id/24652/
Authors:Butschak, G., Karsten, U., Schelhaas, U., Ott, H., Emmendoerffer, A., Niemeyer, B., Helmholz, H., Goletz, S.
Year:2006
In: International Immunopharmacology
Volume:6
Issue:9
Pages:1441 - 1450
Publisher:Elsevier, Amsterdam
Type:Journalpaper
ISSN: 1567-5769
Cite as: Butschak, G.; Karsten, U.; Schelhaas, U.; Ott, H.; Emmendoerffer, A.; Niemeyer, B.; Helmholz, H.; Goletz, S.: Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococus fascians. In: International Immunopharmacology. Vol. 6 (2006) 9, 1441 - 1450. (DOI: 10.1016/j.intimp.2006.04.010)